Effects of breviscapine on protein expression of c-fos,c-jun in glomerular mesangial cells cultured under high glucose conditions
- VernacularTitle:灯盏花素对高糖环境肾系膜细胞c-fos、c-jun蛋白表达的影响
- Author:
Tao JIANG
;
Yan GAO
;
Zuying XIONG
;
- Publication Type:Journal Article
- Keywords:
breviscapine;
glomerular mesangial cell;
glucose;
oncogene;
protein kinase C;
collagen Ⅳ
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore effects of protein kinase C (PKC) inhibitor breviscapine on protein expression of c fos?c jun and collagen Ⅳ(C Ⅳ) production in rat glomerular mesangial cells (GMC) cultured under high glucose conditions. METHODS Rat GMCs were cultured under normal glucose, high glucose or high glucose and breviscapine. After 24 h, 48 h or 1 wk, protein expression of c fos?c jun, C Ⅳ and PKC activity were measured. RESULTS Increased protein expression of c fos?c jun, C Ⅳ and PKC activity were observed under high glucose compared to normal glucose. All the above mentioned effects of high glucose were inhibited completely or partly by breviscapine. CONCLUSION High glucose may increase protein expression of c fos?c jun and C Ⅳ production mediated by PKC activation in cultured GMCs. PKC inhibitor breviscapine may ameliorate these abnormalities efficiently.
