Effect of allicin on the regulation of VEGF mRNA expression in human hepatocellular carcinona cells
- VernacularTitle:大蒜素抑制人肝癌细胞中VEGF mRNA表达的研究
- Author:
Yanjing GAO
;
Mengbiao YUAN
;
Hua XIN
;
Qian WANG
;
Honglian SHAO
;
- Publication Type:Journal Article
- Keywords:
phosphorothioate oligodeoxynucleotides;
hepatocellular carcinoma cells;
reverse transcriptase polymerase chain reaction;
vascular endothelial growth factor;
allicin
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effect of allicin on the regulation of VEGF mRNA expression in human hepatocellular carcinoma cells. METHODS Hepatocellular carcinoma cells were treated with the concentration 10 ?g?L -1 allicin in culture medium,and then the relative VEGF mRNA level at 8 h in human hepatocellular carcinoma cells was evaluated by reverse transcriptase polymerase chain reaction using HPRT(hypoxanthine phosphoribosyltransferase)as an internal control standard. RESULTS The expression of VEGF gene mRNA was inhibited obviously by allicin. Compared with control group, the relative expression level of VEGF gene mRNA was decreased by about 66 36%( P
