Expression of chimeric antigen of HCV with multiple immunodominant epitopes in E .coli and its application for serological tests
- VernacularTitle:丙型肝炎病毒优势表位嵌合抗原在大肠杆菌中的表达及其在血清学检测中的应用
- Author:
Hongyan SUN
;
Quanli WANG
;
Zhiyan DU
;
ETAL
;
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
Chimeric antigen;
Escherichia coli
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective ToexpresschimericantigenofHCVwithmultipleimmunodominantepitopes inE .coliforimprovingthequalityofreagentsforHCVscreening .Methods Thegenefragmentencoding HCVchimericantigenwasobtainedbymolecularcloningmethodandclonedintopQE 30 plasmidforex pressioninE .coliM 15 .TheexpressedchimericantigenwaspurifiedbyNi NTAandcoatedonELISA platestoanalyzeitssensitivityandspecificity .Results Thechimericantigenof 5 30 0 0washighlyex pressed .ELISAassayofserumsamples (including 18HCVpositiveand 17negativesera)indicatedthatthe HCVchimericantigenhadhighsensitivityandspecificity .Conclusions ChimericantigenofHCVwith typicalimmunodominantepitopescanbeusedtodevelopgoodreagentsforHCVimmunoassay .
