Measurement of intestinal permeability in normal Chinese volunteers
- VernacularTitle:健康国人肠粘膜通透性的检测
- Author:
Xiaohua JIANG
;
Ning LI
;
Fangnan LIU
;
Yuanxin LI
;
Jieshou LI
;
- Publication Type:Journal Article
- Keywords:
Intestinal permeability;
Lactulose;
Mannitol;
HPLC
- From:
Parenteral & Enteral Nutrition
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To establish a confident and sensible method of measuring lactulose(L) and mannitol(M) in human urine, and to research lactulose and mannitol excretion ratio of urine in normal Chinese volunteers in 6 hours after taking lactulose and mannitol. Methods:20 healthy volunteers took 10g of lactulose and 5g of mannitol, and 6 hours of urine was collected. The L and M concentration was analyzed by HPLC RI. The excretion of L and M and the L/M ratio was calculated. Results:The excretion of L and M in the urine was 5.74%?0.58% (0.65 %~11.47%) and 0.132%?0.012%( 0.005 %~0.206%),while the L/M ratio was 0.028? 0.010 (0.013~0.051). Conclusions: HPLC is a kind of confident and sensible method to analyze L and M concentration. The L/M ratio can reflect the intestinal mucosal permeability and can be used to monitor intestinal barrier function in clinical practice.
