EFFECT OF LYCIUM BARBARUM POLYSACCHARIDE ON APOPTOSIS OF HUMAN LEUKEMIA CELLS
- VernacularTitle:枸杞多糖对人白血病细胞株凋亡的影响
- Author:
Lu GAN
;
Jianhua WANG
;
Qiong LUO
;
Shenghua ZHANG
;
- Publication Type:Journal Article
- Keywords:
Lycium barbarum polysaccharide;
human leukemia cells;
anticancer;
cell apoptosis
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of LBP X on apoptosis of human leukemia HL 60 cells. Methods: The inhibitory effect of LBP X on growth of HL 60 cells was assayed by MTT method. The membrance fludity was determined with DPH fluorigenic labeling technique. HL 60 cells were stained with Hoechst 33 258 for nuclear morphology analysis. Agarose gel electrophoresis and flow cytometry were used to analyze apoptosis qualitatively and quantitatively. Results: 20-1 000mg/L LBP X inhibited the growth of HL 60 cells in dose dependent manner and the membrance fludity decreased. The nuclei of HL 60 cells treated with LBP X for 48 h shrinked, condensed and cleaved. Agarose gel electrophoresis of DNA from cells treated with LBP X revealed "DNA ladder". HL 60 cells exposed to LBP X showed apoptotic peaks. Conclusion: LBP X can induce apoptosis in human leukemia HL 60 cells.
