rIL-2,TNF-?,IFN-? and anti-CD3/anti-glioma bispecific antibodies cooper atively enhancing T lymphocytes' cytotoxicity against human glioma cells
- VernacularTitle:rIL-2、TNF-?、IFN-?与抗CD3-抗胶质瘤双特异性抗体的协同细胞毒作用
- Author:
Meiqing LOU
;
Yicheng LU
;
Wenzhong WANG
;
Qian SHEN
;
Qiang HUANG
;
Cheng ZHU
;
Shimin YANG
;
Liying LU
;
- Publication Type:Journal Article
- Keywords:
antibodies,bispecific;
glioma;
cytotoxicity,immunologic;
interleukin 2;
tumor necrosis factor;
interferon typeⅡ;
gene therapy
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: : To observe whether cytokines rIL 2,TNF ?,I FN ? and anti CD3/anti glioma bispecific antibody(BsAb) can work coordinately, and to investigate how to further enhance cytotoxicity of T lymphocyte against human glioma cells by BsAb. Methods: There were 12 groups,contr ast method were used to analyze the effect of cytokines rIL 2,TNF ?,IFN ? to cytoxicity directed by BsAb by single and combined experiments. Cytotoxicity was assayed by standard 18 h 3H TdR incorporation release. Resul ts: rIL 2,TNF ?,IFN ? and BsAb could cooperatively enhance the cy totoxicity of effect cells( P
