The effect of glutamate on cytosolic Ca~(2+) concentration and Ca~(2+)influx induced by ATP in cultured calf middle cerebral artery smooth muscle cells
- VernacularTitle:谷氨酸对牛大脑中动脉平滑肌细胞胞浆静息Ca~(2+)和ATP触发的Ca~(2+)内流的影响
- Author:
Quan YI
;
Yongyuan GUAN
;
Hua HE
;
- Publication Type:Journal Article
- Keywords:
middle cerebral artery smooth muscle;
cells;
Ca 2+ influx;
ATP;
glutamate
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate if glutamate has direct effects on both resting [Ca 2+ ] i and Ca 2+ influx induced by ATP in cultured calf middle artery cerebral smooth muscle cells. METHODS The effect of glutamate on [Ca 2+ ] i was determined with Fura2 fluorescence technique. RESULTS Glutamate(10～200 ?mol?L -1 )didnot elevate the resting [Ca 2+ ] i level and in the concentrations of 10～400 ?mol?L -1 failed to affect increase and decrese the Ca 2+ influx induced by ATP. CONCLUSION Glutamate hasn't directly effects on both resting [Ca 2+ ] i; and Ca 2+ influx in middle cerebral artery smooth muscle cells. Glutamate is unlikely to participate in regulation of cerebrovascular tention as a direct effector.
