Effects of Ternatolide on expression granulysin mRNAin the peripheral blood lymphocytes patients with mycobacterium tuberculosis
- VernacularTitle:小毛莨内酯对结核病人颗粒裂解肽基因表达的作用
- Author:
Li ZHAN
;
Huacheng DAI
;
Zhiping YANG
;
Zhuwen YI
;
Ping SHU
;
Shiming CHENG
;
- Publication Type:Journal Article
- Keywords:
Radix Ranunculi Ternati;
Ternatolide;
Granulysin;
Mycobacterium tuberculosis;
Drug resistance;
QC RT PCR
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of ternatolide(tern) on expression of granulysin mRNA in the activated peripheral blood lymphocytes(PBLS) of the adults and children with pulmonary Mycobacterium tuberculosis(PMTB). METHODS Using competitive quantitative reverse transcription polymerase chain reaction(QC TR PCR)analyze granulysin gene expression in the PBLs. RESULTS The dosage of 100 mg?L -1 , 200mg?L -1 of ternatolide significantly enhanced the granulysin mRNA experession in PBLs in vitro stimulated phytohemagglutinin (PHA) from adults and children with PMTB (P0 05). CONCLUSION The molecular mechanism of tern. against intracellular pathogens might be associated with the inducting highly level on the expression of granulysin mRNA, a anti bacterial peptide in activated human cytotoxic lymphocytes.
