Anti-oxidation effects of pyrrolin nitroxides and derivatives in tissues and red blood cell from rats in vitro
- VernacularTitle:吡咯啉氮氧自由基及衍生物抗大鼠不同组织脂质过氧化损伤
- Author:
Shufang WANG
;
Qin WANG
;
Jing PAN
;
Yan ZHANG
;
Ziyi ZHANG
;
- Publication Type:Journal Article
- Keywords:
nitroxides;
lipoperoxidation;
?OH free radicals;
malondialdehyde(MDA);
fenton's reaction
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the anti oxidation activities of new systhesized nitroxides in liver, liver mitochondria and RBC from rats and in egg phospholipid. METHODS The homogenates of liver, liver mitochondria from rats and the suspensions of egg phospholipid were used to determine malondialdehyde (MDA) formation induced by Fe 2+ Vit C system using TBA colorimetric method. H 2O 2 caused hemolysis was measured spectrometrically. Superoxide anion was assayed spectrometrically. RESULTS Nitroxides A, B with one active group (NO?) could inhibit MDA generation caused by ?OH generation system significantly, antagonized hemolysis induced by H 2O 2, but did not affect O ? 2 formation; Nitroxide C with two active group (NO?) possessed similar potent anti lipoperoxidative activities,IC 50
