The effects of propofol and thiopental on Ca~(2+) mobilization in cultured rat ventricular myocytes
- VernacularTitle:异丙酚和硫喷妥钠对大鼠心肌细胞钙离子移动的影响
- Author:
Ying ZHANG
;
Xinliang ZHUANG
;
Shitong LI
;
Guohui XU
;
- Publication Type:Journal Article
- Keywords:
propofol;
thiopental;
ventricular myocytes;
calcium
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To elucidate the direct effects of propofol and thiopental on cardiac contraction by measuring intracellular Ca 2+ concentration change in cultured rat ventricular myocytes. METHOD Ventricular myocytes of neonatal rats were obtained by enzymatic digestion with typsin and were cultured for 4～5 days, then were loaded with Fluo 3AM, a new Ca 2+ indicator. The effects of propofol (50, 250 ?mol?L -1 ) or thiopental (100, 500 ?mol?L -1 ) on changes of intracellular Ca 2+ fluorescent intensity induced by KCl, isoprenaline or caffeine were investigated. RESULTS Both intravenous anesthetics caused dose dependent inhibit Ca 2+ transsarcolemmal influx induced by KCl and isoprenaline, peak Ca 2+ fluorescent intensity decreased. The inhibitory effect of thiopental was more than propofol at equiopotency. Neither anesthetics altered the amount of Ca 2+ release from intracellular stores in response to caffeine. CONCLUSION The negative inotropic effects of propofol and thiopental, at least in part, may be mediated by a decrease Ca 2+ transsarcolemmal influx though voltage gated and receptor gated calcium channel, but it is not relate to sarcoplasmic reticulum Ca 2+ release.
