ENHANCEMENT OF RESISTANCE OF HUMAN BONE MARROW CELLS TO ANTICANCER AGENTS BY mdrl GENE TRANSFECTION
- VernacularTitle:多药耐药基因的人骨髓细胞转染及其对抗癌药的抗性增强作用
- Author:
Liang′An CHEN
;
Youning LIU
;
- Publication Type:Journal Article
- Keywords:
mdrl gene;
human bone marrow cells
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the possibility to enhance resistance of human bone marrow cells to anticancer agents by transfection of mdrl gene. We transferred mdrl gene into human bone marrow cells with plasmid pHaMDR1/A containing human mdrl cDNA by Lipofectin.Pgp,mdrl gene expression product was detected by both flow cytometry and immunohistochemistry.Also,Pgp function was tested by efflux study using rhodamine.The resistance of human bone marrow cells to anticancer agents was assayed by color forming unit culture after exposure to Vincristine,Daunomycin, and Vp16.The results showed that mdrl gene was successfully transferred into human bone marrow cells, and it expressed.Biological activity of Pgp was confirmed by efflux study using rhodamine.Transferred human bone marrow cells possessed resistance to anticancer agents.It suggested that transfection of mdrl gene can increase resistance of human bone marrow cells to anticancer agents.
