Isolation, purification and content determination of melittin from bee venom
- VernacularTitle:蜜蜂毒中蜂毒素的分离纯化方法及含量测定
- Author:
Ling LIU
;
Changquan LING
;
Jinhong HU
;
Xueqiang HUANG
;
- Publication Type:Journal Article
- Keywords:
melittin;
chromatography,gel;
electrophoresis,polyacrylamidegel;
chromatography, high pressure liquid
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and purify melittin from bee venom and to establish an SDS PAGE and HPLC method for the determination of melittin content.Methods:Melittin was isolated and purified from bee venom by gel filtration on Sephadex G 25,Sephadex G 50 and Sephadex G 75.Homogenicity and molecular weight of melittin were determined by SDS PAGE and HPLC.Results:The sample was a single peptide chain with a molecular mass of about 3 000 dalton as revealed by SDS PAGE.The average recovery was 95.97% and RSD was 1.07%.Conclusion:Melittin prepared by a combination of gel filtration has high purity.The method for the determination of melittin with SDS PAGE and HPLC is accurate,reliable and simple. [
