Investigation of androgen receptor gene expression in human peripheral leukocytes
- VernacularTitle:人外周血白细胞雄激素受体基因表达的研究
- Author:
Junjie ZOU
;
Zhimin LIU
;
Quan SHANG
;
Yonglian ZHANG
;
- Publication Type:Journal Article
- Keywords:
receptors,androgen;
blotting, Northern;
polymerasechain reaction;
leukocytes
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate androgen receptor (AR) mRNA expression in human peripheral leukocytes. Methods: Total RNA was isolated from peripheral leukocytes of healthy men and women, AR mRNA was determined by RT PCR and Northern blot. Results: An AR cDNA fragment of the expected size of 390 bp was determined by RT PCR. 9.4 kb AR mRNA was detected by Northern blot, which was consistent with AR mRNA in human prostate reported by Lubahn et al . Conclusion:AR mRNA expresses in human peripheral leukocytes, this provides basis for studying the effect of androgen on the function of the human peripheral leukocytes and the changes of AR in pathological stages. [
