Effects of recombinant human hepatocyte growth factor on proliferation and extracellular matrix synthesis in autosomal dominant polycystic kidney disease cyst lining epithelial cells in vitro
- VernacularTitle:肝细胞生长因子对多囊肾病囊肿衬里上皮细胞增殖及细胞外基质合成的影响
- Author:
Aihong YUAN
;
Changlin MEI
;
- Publication Type:Journal Article
- Keywords:
hepatocyte growth factor;
recombination genetic;
kidney,polycystic,autosomal dominant;
cyst lining epithelial cells;
extracellular matrix
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of recombinant human hepatocyte growth factor (rhHGF) on proliferation and extracellular matrix synthesis of autosomal dominant polycystic kidney disease (ADPKD) cyst lining epithelial cells in vitro . Methods: The effects of different concentration rhHGF (0.5,1,2.5,5 ng/ml)in 48 h and the optimal concentration rhHGF of different time (24,48,72 h ) on proliferation of ADPKD cyst lining epithelial cell lines were observed by the incorporation of 3H TdR, and synthesis of collagen and laminin were respectively observed by the incorporation of 3H proline and radioimmunoassay. Results: rhHGF stimulated the proliferation of ADPKD cyst lining epithelial cells and synthesis of collagen and laminin,the optimal concentration and time of rhHGF were 1 ng/ml and 48 h. Conclusion: rhHGF can significantly stimulate ADPKD cyst lining epithelial cells proliferation and extracellular matrix synthesis in vitro . [
