The clone and expression of human THANK gene
- VernacularTitle:人THANK基因的克隆和表达
- Author:
Dong WU
;
Feng SHEN
;
Yonghua LOU
;
Yuping ZHU
;
Binghua JIAO
;
Mengchao WU
;
- Publication Type:Journal Article
- Keywords:
genes, THANK;
ploymerase chain reaction;
cloning,molecular
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone THANK gene and express its extracelluar fragment. Methods: Using RNA isolated from HL 60 cell lines, THANK cDNA was amplified by RT PCR. The fragment was linked to pMD18 T vector and sequenced, and then the extracellular fragment of THANK was subcloned into pET vector and THANK protein expression was induced.Results: A 858 bp DNA fragment was amplified and the cDNA sequence was identical with the published sequence encoding THANK gene. Western blot showed that THANK protein with a relative molecular weight of 2.6?10 4 was expressed. Conclusion: Human THANK gene was cloned and expressed successfully, which provides a base of further research of THANK gene. [
