Study of differentially expressed genes in laryngeal squamous cell carcinoma by cDNA microarray
- VernacularTitle:基因表达谱芯片发现36条喉鳞癌相关基因
- Author:
Juxiang CHEN
;
Jingping FAN
;
Kang YING
;
Aihua SUN
;
Jianchun LIAO
;
Rong TANG
;
Yan HUANG
;
Yao LI
;
Yi XIE
;
Yumin MAO
- Publication Type:Journal Article
- Keywords:
laryngeal neoplasms;
squamous cell carcinoma;
gene expression;
DNA microarray
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen for the differentially expressed genes in laryngeal squamous cell carcinoma and normal laryngeal tissue using cDNA microarray. Methods: The PCR products of 4 096 genes were spotted on a chemical material coated glass plates in array. The DNAs were then fixed on the glass plate by a serial of treatments. The total RNAs were isolated from the tissues, and then were purified to mRNAs by Oligotex. Both the mRNAs from the laryngeal squamous cell carcinoma and normal tissue were reversely transcribed to cDNAs with the incorporations of fluorescent dUTP, for preparing the hybridization probes. The mixed probes were then hybridized to the cDNA microarray. After high stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed the differences between 2 tissues. Results: Among the 4 096 target genes, there were 36(0.88%) genes whose expression levels differed between the carcinoma and normal tissues in all 4 cases. Bioinformatical analysis of those genes had been performed. Conclusion: DNA microarray technology is an effective technique in screening for differentially expressed genes between 2 different kinds of tissue. Further analysis of the obtained genes will help to understand the molecular mechanism of malignant carcinoma. [
