Expression changes of TNF-? mRNA after brain explosive injury in dogs
- VernacularTitle:狗颅脑爆炸伤后脑组织中TNF-?mRNA表达的变化
- Author:
Lijun HOU
;
Guangji ZHANG
;
Yicheng LU
;
Cheng ZHU
;
Yinqiu LIU
;
Bingcang LI
;
Xinan LAI
;
Shuguang LI
;
Wei LI
;
Wenqin CAI
- Publication Type:Journal Article
- Keywords:
brain injuries;
gunshot wound;
tumor necrosis factor
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression changes of TNF ? mRNA after brain explosive injury in dogs. Methods: With the brain explosive injury model we had developed, nuclei acid in situ hybridization with an antisense RNA probe and computer image analysis were used to detect the dynamic changes of TNF ? mRNA in the cerebral cortex, hippocampus, hypothalamus, and up brain stem after brain injury. Results: The expression of TNF ? mRNA could be found in several regions of normal dog's brain tissues, and the expression became more obvious and widespread following explosive injury to the brain. In all of the brain regions, the expression changes of cerebral contussive area and hippocampus were more significant. TNF ? mRNA expression increased significantly at 30 min and reached maximum at 1 h after the explosive injury, and then decreased gradually, which was higher than normal's. More expression were detected in the ipsilateral hemisphere than contralateral hemisphere. Conclusion: The expression of TNF ? mRNA in basal condition implicate that TNF ? may play an important role in neural function. It is suggested that TNF ? may play an important role in traumatic brain injury. [
