The change of intracelluar Ca~(2+) level regulated by endothelial protein-activated by acetylcholine in cultured bovine aorta endothelia cell
- VernacularTitle:牛主动脉内皮细胞乙酰胆碱激活蛋白介导的胞内钙离子变化
- Author:
Lili WANG
;
Jianhua DING
;
Hai WANG
;
- Publication Type:Journal Article
- Keywords:
Ach;
[Ca 2+ ] i;
endothelium;
cell culture
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To examine the change of intracelluar Ca 2+ level regulated by EPA in bovine aorta endothelium of different stages of culture and determine the time that cultured endothelium loses functional EPA. METHODS As fluorescence probe, Fluo 3 and Fura 2 were used in assaying [Ca 2+ ] i in endothelium by confocal microscopy and fluorophotometry. RESULTS Ach could activate EPA and elicite the [Ca 2+ ] i change of endothelium in certain periods of culture, especially in primary stage of cul ture. The rises of [Ca 2+ ] i exhibited as oscillation characterized by iso timing and immediate tolerance. The functional EPA was lost in passage 13. CONCLUSION EPA can be activated by Ach, which mediated the rise of intracelluar Ca 2+ in bovine aorta endothelia cell. This character of EPA is lost during passaging.
