Paclitaxel-induced apoptosis corelated with down-regulation of Bcl-2 and up-regulation of bax in MCF-7 breast cancer cell
- VernacularTitle:紫杉醇诱导MCF-7凋亡与bcl-2及bax的关系
- Author:
Gang MENG
;
Qinglin LI
;
Zhiwu CHEN
;
Chuangeng MA
;
- Publication Type:Journal Article
- Keywords:
paclitaxel;
cisplatin;
MCF 7;
apoptosis;
bax;
bcl 2
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effect of paclitaxel and cisplatin on apoptosis of MCF 7 and its mechanism. METHODS The apoptotic cells were detected by TUNEL method and the expression of Bcl 2 and bax were examined using immunohistochemistry S P method in different time. RESULTS Cisplatin resulted in the death of MCF 7, but did not cause the apoptois of MCF 7. Paclitaxel caused time dependent and concentration dependent increases in the apoptotic cells. Treatment of MCF 7 cells with paclitaxel resulted in time and concentration dependent decreases in bcl 2 and increased in bax proteins. CONCLUSION The apoptosis induced by paclitaxel is related to the increase of bax protein and the decrease of bcl 2 protein.
