Cancer promoting effects of N-nitrosodiethylamine (DEN) and 2-acetylaminofluorene (2-AAF) with medium term rat liver bioassay
- VernacularTitle:应用大鼠中期肝癌试验研究DEN和2-AFF的促癌作用
- Author:
Xingchu GU
;
Beili QIAN
;
Huijuan ZHANG
;
Jianqiang JIANG
;
Huiying YE
;
Yuzhen ZHU
;
Jicheng WANG
;
- Publication Type:Journal Article
- Keywords:
rat,carcinogenisity test;
N nitrosodiethylamine (DEN);
2 Acetylaminofluorene (2 AAF);
Glutathione S Transferase placental form (GST P);
partial hepatectomy;
immunohistochemistry;
image analysis
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the cancer promoting effects of N nitrosodiethylamine (DEN) and 2 Acetylaminofluorene (2 AAF). METHODS Medium Term Rat Liver Bioassay (MTRLB). Male SD rats were initially given a single dose (200 mg?kg -1 ) of DEN ip and starting 2 weeks later, were treated with 10, 33 and 100 ppm DEN in drinking water, or with 2 2, 6 6 and 22 mg?kg -1 2 AAF by gavage for 6 weeks. All rats were subjected to two thirds partial hepatectomy at week 3 and killed at the end of week 8. Carcinogenic potential was scored by comparing the numbers and areas in induced glutathione S transferase placental form (GST P) positive foci in the liver with those of corresponding control group given DEN alone. RESULTS Both DEN and 2 AAF caused the increases of the numbers and areas of GST P positive foci in the liver, and showed dose response relationship. CONCLUSION Both DEN and 2 AAF shows cancer promoting effects, and MTRLB was a convenient, economical and effective tool to study the cancer promoting effects of test chemicals.
