Hemin upregulates the expression of the inducible heme oxygenase gene in the lung of hypoxic rat
- VernacularTitle:血晶素促进缺氧大鼠肺组织诱导型血红素氧合酶基因的表达
- Author:
Guohua ZHEN
;
Zhenxiang ZHANG
;
Yongjian XU
;
- Publication Type:Journal Article
- Keywords:
hemin;
heme oxygenase;
carbon monoxide;
pulmonary hypertension;
hypoxia
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effect of Hemin on the expression of inducible heme oxygenase (HO 1) in hypoxic rat lung tissue. METHODS The rat model of hypoxic pulmonary hypertension was recreated by intermittent normal pressure hypoxia (10% O 2). The following assays were carried out: reverse transcriptase polymerase chain reaction (RT PCR) were performed to determine the level of HO 1 mRNA in rat lung tissue, double wave length spectrophotometry was used to evaluate the quantity of COHb in arterial blood, cardiac catheterization was used to measure the right ventricular systolic pressure (RVSP). RESULTS After exposure to hypoxic enviorenment for 15 days, the level of HO 1 mRNA and COHb were higher than those of the control group ( P
