Anaesthesia of thiopental sodium on nitride oxide synthases activity and nitride oxide dynamic change of rat brain
- VernacularTitle:硫喷妥钠麻醉前后鼠脑NOS活性、NO的动态变化
- Author:
Lin ZHANG
;
Tijun DAI
;
Hongliang LIU
;
Jing MENG
;
Shiming DUAN
;
- Publication Type:Journal Article
- Keywords:
thiopental sodium;
NO;
NOS;
brain
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of thiopental sodium on nitric oxide synthases (NOS) activity and nitride oxide (NO) in different rats cerebral synaptic membrane. METHODS Forty SD rats were divided randomly into five groups. The animals were injected introperitoneally (ip)thiopental sodium 30 mg?kg -1 or normal saline 10 ml?kg -1 (control group ) respectively. These rats were immediately decapitated before (induction group) and after (anaesthetic group) having disappeared righting reflex, and when righting reflex appeared again (recovery group), and completely conscious (awake group). In order to prepare synapsis, brain tissues were dissected on ice, then homogenized and centrifuged. NOS activity and NO was estimated by spectrophotometry. RESULTS Thiopental sodium 30 mg?kg -1 ip significantly inhibited NOS activity of cortex, brain stem and synaptic membrane as compared with that of normal saline group( P
