Effect of N-acetylcysteine on NF-?B in lung tissue and BALF of rats undergoing acute and chronic smoking
- VernacularTitle:N-乙酰半胱氨酸对急慢性吸烟大鼠肺组织和肺泡灌洗液中核因子-?B表达的影响
- Author:
Hongying MO
;
Nanshan ZHONG
;
Jingping ZHENG
;
Qicai LONG
;
- Publication Type:Journal Article
- Keywords:
N acetylcysteine;
nuclear factor ?B;
smoking;
rats
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To assess the effect and mechanism of N acetylcysteine (NAC, antioxidant) on nuclear factor ?B (NF ?B) in lung tissue and bronhial alveolar lavage fluid(BALF) of rats undergoing acute and chronic smoking. Methods All Wistar rats were divided randomly into three groups: control group, smoking group and intervention group. The latter two groups were applied to observing NF ?B expression during different periods (1, 2, 7, 14, 60d). Western Blot assay was used to analyze the activation of NF ?B. Results The first and second day the expression of NF ?B in lung tissue ( 36.9 ? 8.1 and 36.9 ? 8.0 )and in BALF( 24.0 ? 6.1 and 21.2 ? 5.8 ) rose significantly comparing with that in the control groups ( 7.3 ? 2.8 and 5.7 ? 2.6 )respectively (P
