Establishment of a mouse hepatocellular carcinoma cell line producing mMIP-1? chemokines and the tomorigenicity of mMIP-1? transfected Hepa1-6
- VernacularTitle:分泌mMIP-1?趋化因子的小鼠肝癌细胞株的建立及其体内致瘤性
- Author:
Linhua QIN
;
Lixin WEI
;
Qing YANG
;
Yan LU
;
Mengchao WU
;
Yajun GUO
;
- Publication Type:Journal Article
- Keywords:
chemokines;
MIP 1?;
liver neoplasms;
immunity;
gene therapy
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a mouse hepatocellular carcinoma cell line that can produce mMIP 1? and to evaluate the possibility of cancer gene therapy by mMIP 1?. Methods: mMIP 1? cDNA was cloned into retrovirus vector pBabe puro and pBabe puro mMIP 1? was constructed, then pBabe puro mMIP 1? was used to transfect packaging cells, anti puromycin cells was proliferated, the supernatant was used to infect hepa1 6, the anti puromycin clone (hepa1 6 mMIP 1?) and hepa1 6 were analysed for the expression of mMIP 1? mRNA and protein by RT PCR and immunohistochemistry respectively. The growth curve of hepa1 6 and hepa1 6 mMIP 1? was drawn. The chemotaxis of mMIP 1? produced by hepa1 6 mMIP 1? to mouse spleen cells was observed on agarose gel. C57B/L mouse was inoculated with the tumor cell and the tumorigenicity was studied. Results: Recombinant retrovirus vector pBabe puro mMIP 1? with mMIP 1? cDNA was constructed. Hepa1 6 did not produce mMIP 1? mRNA and protein, while hepa1 6 mMIP 1? could produce mMIP 1? mRNA and protein. The growth curve of hepa1 6 and hepa1 6 mMIP 1? showed no difference. The chemotaxis of mMIP 1? produced by hepa1 6 mMIP 1? to mouse spleen cells was observed. The tumorigenicity was reduced. Conclusion: A mouse hepatocellular carcinoma Hepa1 6 mMIP 1? is established and mMIP 1? can affect the tumorigenecity of hepa1 6.
