Experimental studies on methionine dependence of human primary gastric cancer cells
- VernacularTitle:人胃癌原代细胞的甲硫氨酸依赖性研究
- Author:
Weixin CAO
;
Jingmin OU
;
Haoran YIN
;
Zhenggang ZHU
;
Min YAN
;
- Publication Type:Journal Article
- Keywords:
Methionine;
Dependence;
Primary gastric cancer cell;
Cell culture
- From:
Parenteral & Enteral Nutrition
1997;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To study the methionine dependence (Met-dependence) of human primary gastric cancer cells in vitro when Met in the culture medicine was replaced by its precussor homocysteine(Hcy). Methods:Fresh and sterile gaotric cancer samples and normal gastric mucosa were managed into single-cell suspension and were cultured in the Met -Hcy + and Met\++ Hcy\+- medium separately.proliferation of these cells in the two different culture media was examined by cell counter and flow cytometry (FCM). Results:In the Met\++Hcy\+- medium,the growth of primary gastric cancer cells was inhibited,manifesting that the percentage of G 0G 1 tumor cells decreased and that of the S phase increased signiflcantly.However,normal gastric mucosal cells grew well and their cell cycles had no change. Conclusions:Human primary gastric cancer cells are Met dependent in vitro whereas normal gastric mucosal cells have no similar results.
