EFFECTS OF ?-TOCOPHEROL AND ?-CAROTENE ON OXIDATION OF LOW DENSITY LIPOPROTEIN BY MONOCYTE DERIVED MACROPHAGES
- VernacularTitle:维生素E和?胡萝卜素对巨噬细胞氧化修饰低密度脂蛋白的影响
- Author:
Xiaolong CHENG
;
Yongping CUI
;
Yanqiu CHEN
;
Xizhong ZHANG
;
- Publication Type:Journal Article
- Keywords:
vitamin E;
? carotene;
macrophage;
LDL;
oxidation
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To examine the effects of ? carotene and ? tocopherol on LDL oxidation by monocyte derived macrophages from SD rats (in DMEM medium). [WT5FZ]Methods: Macrophages were incubated with LDL in DMEM in which freshly dissoved VE (40,100,200 ?mol/L) and ?C (0.5,1.0,2.0 ?mol/L) had been added. After incubating for 24 h, the mediums were centrifuged to determine TBARS and lipofusin and dienes. [WT5FZ]Results: Supplementation of ? tocopherol significantly decreased TBARS, lipofusin and conjugated dienes production and electrophoretic mobility. Supplementation of ? carotene at 0.5 ?mol/L significantly decreased TBARS, lipofusin and conjugated dienes production and electrophoretic mobility, but other levels showed no effect. Increasing ? carotene concentration to 2.0 ?mol/L in the system increased the lipofusin production compared with control group. ? tocopherol at 200 ?mol/L and ? carotene at 0.5 ?mol/L were the most effective in all dose groups. [WT5FZ]Conclusion: Cell mediated oxidation of LDL can be regulated by antioxidants such as ? tocopherol and ? carotene.
