Effects of probucol on the NF-?B-like activity of aortic smooth muscle cell in vitro
- VernacularTitle:普罗布考对体外培养的主动脉平滑肌细胞NF-?B活性的影响
- Author:
Jiangtao GU
;
Zonggui WU
;
Qian SHEN
;
Yulian XU
;
- Publication Type:Journal Article
- Keywords:
probucol;
hydrogen perioxide;
vascular smooth muscle cells;
nuclear factor ?B
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the inhibitory effects of probucol on nuclear factor ?B (NF ?B) like activity of vascular smooth muscle cells(VSMCs). Methods:The effects of probucol and H 2O 2 on NF ?B like activity of VSMCs were investigated by electrophoretic mobility shift assay(EMSA). Results:NF ?B activation of VSMCs could be induced by H 2O 2 or new born calf serum(NCS) for 72 h, the gray measure of the protein bindings measured through computer scanning was 63.9 and 46 6 respectively. NF ?B activity of VSMCs stimulated by H 2O 2 or NCS might be prevented by 100 ?mol/L probucol, the inhibitory rate was 37.1%, 14.8% respectively. Conclusion:probucol can inhibit NF ?B activity of VSMCs stimulated by NCS or H 2O 2. These effects of probucol might help to reduce development of atheroclerosis and restenosis after percutaneous transluminal coronary angioplasty. [
