Effect and mechanism of ?_(1B)-adrenoceptor on cell growth in DDT1 MF-2
- VernacularTitle:激动?_(1B)-肾上腺素受体对DDT1 MF-2细胞生长的刺激作用及其途径
- Author:
Benbo GAO
;
Beilei LEI
;
Qide HAN
;
- Publication Type:Journal Article
- Keywords:
adrenergic receptor;
subtype;
DDT1 MF2 cell;
cell growth;
signal transduction;
protein kinase
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM DDT1 MF 2 hamster smooth muscle cells were used to investigate the role of ? 1B adrenoceptor (AR) in the cell proliferation and its signaling pathway. METHODS DNA synthesis was measured by [ 3H]thymidine (TdR) incorporation and the cell cycle was determined by flow cytometry. The actions of several inhibitors and activators of second messenger on NE induced DNA synthesis were investigated. RESULTS NE (0 1～1 ?mol?L -1 ) elicited significant concentration dependent stimulation of DDT1 MF 2 cell proliferation. The proliferative effect caused by ? 1B AR was blocked by PLC inhibitor (U73122, 10 ?mol?L -1 ), Ca 2+ /ATPase inhibitor (cyclopiazonic acid, 10 ?mol?L -1 ), intracellular Ca 2+ chelator (BAPTA/AM, 10 ?mol?L -1 ), PKC inhibitors (RO 31 8220, 0 1 ?mol?L -1 and calphostin C, 0 1 ?mol?L -1 ), TK inhibitors (tyrphostin A25, 10 ?mol?L -1 and genistein, 10 ?mol?L -1 ), and MEK1/2 inhibitor (PD 98059, 10 ?mol?L -1 ). CONCLUSION ? 1B AR subtypes stimulate DDT1 MF 2 cells growth and its signal pathway is related to the PLC activation、Ca 2+ release、PKC、TK and ERKs activation.
