The inhibitory effects on the growth of human hepatocellular carcinoma cell and its mechanism by theasinesin
- VernacularTitle:聚酯型儿茶素对人肝癌细胞生长的抑制作用及其机制
- Author:
Huawen XIN
;
Fandian ZENG
;
Bijun XIE
;
- Publication Type:Journal Article
- Keywords:
theasinesin;
carcinoma,hepatocellular;
cell line
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the inhibitory effects on the growth of SMMC 7721 human hepatocellular carcinoma cell line and its mechanism by theasinesin. METHODS MTT assay,colony formation test, 3H TdR, 3H Leu incorporation test, determination of the intracellular cAMP and cGMP level, in situ hybridization were used. RESULTS Theasinesin at 50 mg?L -1 could significantly inhibit the growth and colony formation of SMMC 7721 cells. Theasinesin could strongly inhibit DNA and protein synthesis and increase intracellular cAMP levels. C myc gene expression was significantly decreased and p53 gene increased strongly in a dose dependent manner. CONCLUSION Theasinesin demonstrates significant inhibitory effects on the growth of SMMC 7721 cells. Its mechanisms may involve the inhibition of DNA and protein synthesis ,the elevation of intracellular cAMP levels, inhibition of c myc gene expression and enhancement of p53 gene expression.
