Effect of quateranary ammonium salt derivative (F_2) of haloperidol on calcium in vascular smooth muscle cells
- VernacularTitle:氟哌啶醇季铵盐衍生物对血管平滑肌细胞钙离子的影响
- Author:
Risheng WEI
;
Ganggang SHI
;
Yingna ZHANG
;
- Publication Type:Journal Article
- Keywords:
quateranary ammonium salt of haloperidol;
vascular smooth muscle cells;
laser scanning confocal microscope;
fluo 3 AM;
Ca 2+
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observe the effect of quateranary ammonium salt derivative (F 2) of haloperidol on calcium level in vascular smooth muscle cells (VSMCs) isolated from thoracic aortas of rat. METHODS VSMCs were loaded with Fluo 3 AM, a calcium sensitive fluorescent dye, and [Ca 2+ ] i was determined by the use of laser scanning confocal microscope (LCSM). RESULTS In Ca 2+ (1 26 mmol?L -1 ) bath solution, intracellular calcium fluorescent intensity (FI) in VSMCs was increased when application with 30 mmol?L -1 KCl, and then was inhibited after addition with F 2. The FI of the ASMCs with different concentrations of F 2 (0 1,1,10,100 ?mmol?L -1 )within three minutes were 64%?9%( n =16, P
