Change of ryanodine receptor subtypes expression in pulmonary artery smooth muscle cell from pulmonary hypertensive rats
- VernacularTitle:肺动脉高压大鼠肺动脉平滑肌细胞Ryanodine受体亚型的变化
- Author:
Ying WANG
;
Zhi LI
;
Libin LIU
;
Hua CONG
;
- Publication Type:Journal Article
- Keywords:
ryanodine receptor;
monocrotaline;
pulmonary hypertension;
vascular smooth muscle;
calc$
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the exact subtype of ryanodine receptor (RyR) in pulmonary artery smooth muscle cell(PASMC) and the change of RyR expression from pulmonary hypertensive (PAH) rats. METHODS Rats pre treated with a single intraperitoneal injection of monocrotaline (MCT, 60 mg?kg -1 ) were used to produce PAH animal model and killed 21 d later. The subtype expression of RyR and the change of their mRNA were measured by RT PCR; protein expression of RyR was measured by Western blot in PASMC. RESULTS The PASMC of rats only expressed type ⅡRyR (RyR2). RT PCR showed the RyR2 mRNA concentration of PAH group was higher than that of control; Western blot demonstrated that the RyR2 protein level in PAH group was increased to (170 23?45 34)%. CONCLUSION PASMC only expressed RyR2 subtype and the increment of RyR2 expression may contribute to the pathological mechanism of PAH formation.
