Effects of danshensuan on expression of CD11b, P-selectin, ICAM 1, VCAM-1 and E-slectin in vitro
- VernacularTitle:丹参素对CD11b、P-selectin、ICAM-1、VCAM-1、E-selectin表达的影响
- Author:
Kaiyu JIANG
;
Zhenlun GU
;
Changgeng RUAN
;
- Publication Type:Journal Article
- Keywords:
danshengsuan;
cell adhesion molecules;
flow cytometry;
thrombin;
tumor necrosis factor;
CD11b;
P selection;
ICAM 2;
VCAM 1,E select
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the action of danshengsuan (Dan) on expression of CD11b, P selectin, ICAM 1, VCAM 1 and E slectin in vitro. METHODS Adheshion molecule expression on the cell surface was measured by flow cytometry. RESULTS Treatment of neutrophils with N fomyl met leu phe(fMLP), resulted in a marked increase of CD11b expression. Dan inhibited the effects of fMLP in a concentration dependent manner. Dan did not inhibit the increase of P selectin expression of thrombin actived human blood platelets. Treatment of cultured human umbilical vein endothelial cells (HUVEC) with TNF ? resulted in an increase of ICAM 1, VCAM 1 and E slectin. Dan inhibited the increase of VCAM 1 and E slectin expression, not ICAM 1. CONCLUSION Dan inhibited expression of adhesion molecules CD11b, ICAM 1, VCAM 1 in human neutrophils and HUVEC.
