Effect of GABA_B receports on taurine-regulate release of amino acids from rat cortical synaptosomes
- VernacularTitle:GABA_B受体对牛磺酸调节突触体氨基酸释放的影响
- Author:
Lixiang ZHENG
;
Rencai PENG
;
Hanqing LIU
;
Fusheng WAN
;
- Publication Type:Journal Article
- Keywords:
taurine;
presynaptic GABA;
receptors;
Asp;
Glu;
GABA;
synaptosomes;
rat cor
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM Taurine can regulate Asp、 Glu、 GABA from rat cortical synaptosomes. But its mechanism is unclear. The release regulated effects were investigated through analysis of GABA receptors.METHODS Bicuculline、Phaclofen、Baclofen were added in a Krebs Ringer buffer with resuspended synapotomes.Endogenous Asp、 Glu and GABA release during the 5 min superfusion were measured by high perfusion liquid chromatography using percolumn durivatization with Dans Cl. RESULT Phaclofen,but not bicucullion baclofen, counteracted the inhibition of GABA overflow,although the inhibition of Asp and Glu overflow was not attenuated. CONCLUSION Taurine inhibits the depolarization evoked release of GABA through the activation of presynaptic autoreceptors and taurine also acts on presynaptic sits of Asp Glu nerve terminals to inhibit their evoked release in rat cerebral cotex.
