EFFECT OF ZINC ON THE GENE EXPRESSION OF HEPATIC METALLOTHIONEIN-1 AFTER ISCHEMIA REPERFU- SION INJURY IN RATS
- VernacularTitle:锌对缺血再灌注大鼠肝脏金属硫蛋白-1基因表达的影响
- Author:
Yugang JIANG
;
Changjiang GUO
;
Yuzhang GE
;
Jingyu WEI
;
Donglan WANG
;
Lanxing GAO
;
- Publication Type:Journal Article
- Keywords:
metallothionein 1;
reverse transcription polymerase chain reaction;
hepatic ischemia reperfusin;
zi
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:[WT5BZ]To explore the molecular mechanism of protection of zinc against hepatic ischemia reperfusion injury(HIRI). [WT5HZ]Methods:[WT5BZ]The expression of hepatic metallothionein 1(MT 1)gene and regulation by zinc were determined by RT PCR(reverse transcription polymerase chain reaction)in HIRI rats. [WT5HZ]Results:[WT5BZ]1.Hepatic MT 1 mRNA was expressed in all groups;2.The level of hepatic MT 1 mRNA in HIRI group(ischemia 30 min,reperfusion 90 min)was significantly lower than control.After zinc supplementation,the content of hepatic MT 1 mRNA was increased significantly;3.The hepatic MT 1 expression was also enhanced by zinc in normal rats. [WT5HZ]Conclusion:[WT5BZ]The results of our studies suggest that the regulation of hepatic MT 1 genes by zinc is one of the main ways contributed to the mechanism of protection by zinc in HIRI.
