Expression of IL-1ra mRNA and dysfunction of phagocytosis and intracellular killing of peripheral blood neutrophils in HD patients
- VernacularTitle:维持性血透患者外周血中性粒细胞的IL-1ra基因表达和吞噬杀菌功能
- Author:
Lili GUO
;
Weijie YUAN
;
Zhibin YE
;
- Publication Type:Journal Article
- Keywords:
neutrophils;
hemodialysis;
receptor antagonists, IL 1;
phagocytosis;
antisptics
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the function and IL 1ra gene expression of neutrophils in hemodialysis (HD) patients. Methods: Gene expression was studied by reverse transcribe polymerase chain reaction (RT PCR) from untreated neutrophils in healthy controls and HD patients. Phagocytosis and bactericidal assays decreased in the number of viable extracellular and intracellular bacteria by colony counts. Results: Freshly isolated neutrophils spontaneously expressed icIL 1ra, sIL 1ra mRNA in HD patients. Phagocytosis and intracellular killing of neutrophils in HD patients impaired obviously when exposed to staphylococcus aureus in comparison with healthy controls. Conclusion: Untreated neutrophils in HD patients spontaneously express icIL 1ra and sIL 1ra mRNA, which is consistent with the suppressed phagocytosis and intracellular killing capacity. [
