The Analysis of Interrelationship between Homocysteine and Methylenetetrahydrofolate Reductase Mutation in Patients with Recurrent Spontaneous Abortion.
- Author:
Yoon Sung NAM
;
Kwang Yul CHA
;
Nam Keun KIM
;
Myung Seo KANG
;
Se Hyun KIM
;
Doyeon OH
- Publication Type:Original Article
- Keywords:
Recurrent spontaneous abortion;
Homocysteine;
MTHFR mutation
- MeSH:
Abortion, Spontaneous*;
Female;
Homocysteine*;
Humans;
Hyperhomocysteinemia;
Immunoassay;
Methylenetetrahydrofolate Reductase (NADPH2)*;
Pregnancy
- From:Korean Journal of Fertility and Sterility
2002;29(3):187-194
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To analyze the interrelationship between homocysteine and methylenetetrahydrofolate reductase (MTHFR) mutation in patients with recurrent spontaneous abortion. MATERIAL AND METHOD: Homocysteine and MTHFR mutation were tested by fluorescent polarizing immunoassay and PCR-RFLP method, respectively. RESULTS: In patients with homocysteine level less than 5 mmol/L, there was no case of normal group but there were four cases of heterozygosity and one case of homozygosity. In patients with homocysteine level 5~10 mmol/L, the number of normal, heterozygosity and homozygosity group were eleven, eighteen and eight, respectively. In patients with homocysteine level 10~15 mmol/L, the number of normal, heterozygosity and homozygosity group were four, one and one, respectively. In patients with homocysteine level more than 15 mmol/L, there was no case of normal and heterozygosity group but there were two cases of homozygosity. CONCLUSIONS: Hyperhomocysteinemia due to MTHFR mutation is a cause of recurrent spontaneous abortion. And there was a significant relationship between homocysteine and MTHFR mutation.
