Application of dextran sulfate pretreatment in animal model of umbilical cord blood transplantation
- VernacularTitle:硫酸葡聚糖预处理方案在脐血移植中应用的初步研究
- Author:
Jianqiu WU
;
Zhijun JIN
;
Yingsong XIANG
;
Yunfang YANG
;
Rujun YANG
;
Ming YAO
;
- Publication Type:Journal Article
- Keywords:
hematopoietic stem cell transplantation;
severe combined immunodeficient mice;
dextran sulf
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To better understand homing potentiality of hematopoietic stem cells (HSC) in human umbilial cord blood (UCB) and the mechanism of dextran sulfate (DS) mobilization. Methods: Sub lethally irradiated or DS pretreated severe combined immunodeficient (SCID) mice were transplanted with UCB, which was cryopreserved at -80℃.Human cells in recipient mice were detected by flow cytometry and CFU GM assay from each host organ. Results: In contrast with the controls, engraftment after irradiation or administration of DS resulted in a higher percentage of CD45 +,CD34 +,CD19 + cells produced in SCID mice. While comparison between the experimental groups, higher implantation level was obtained in the former if equivalent donor cells were used in both groups. Conclusion:DS is a safe and effective pretreatment, which can mobilize HSC, but also vocate niches for transplanted HSC homing. [
