Protective immunity induced by DNA vaccine of Cysticercus cellulosae antigen
- VernacularTitle:猪囊尾蚴抗原DNA疫苗诱导的免疫保护效应
- Author:
Dan WU
;
Yingjun GUO
;
Yi LIN
;
Shuhan SUN
;
- Publication Type:Journal Article
- Keywords:
Cysticercus cellulosae;
DNA;
vaccines;
protective immuni$
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the immune response induced by Cysticercus cellulosae protective antigen cC1 DNA vaccine in mice and the protective immunity induced by immunized newborn pigs. Methods: Recombined plasmid p3 cC1 was constructed by inserting full length cC1 cDNA into an eukaryotic expression vector pcDNA3. Mice were injected intramuscularly with the recombined construct. Anti cC1 antibody and IgG 2a in serum were screened by ELISA. Then the protective immunization in pigs was done. Results: The immune response of specific antibody was induced during the 3r week. The highest level was during the 8th week. IgG 2a response was detected during the 2nd week. The higher duration of IgG 2a response induced by DNA vaccine was longlived. The protective rate induced in immunized newborn pigs was 73%. Conclusion: The cC1 DNA vaccine can effectively induce protective immunity in newborn pigs.
