Inhibition of midazolam on macroscopicsodium currents in rat sympathetic neurons
- VernacularTitle:咪唑安定对交感神经元全细胞钠通道电流的抑制作用
- Author:
Ji-Jian ZHENG
;
Xin-Liang ZHUANG
;
Bao-Gang LIU
;
Dong-Ping DU
;
Guo-Hui XU
;
- Publication Type:Journal Article
- Keywords:
midazolam;
sympathetic ganglion;
sodium channel;
patch-clamp
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim The effects of midazolam on the whole-cell sodium currents in rat sympathetic neurons were studied to explore the mechanisms where by midazolam mediates hypotension. Methods Whole-cell patch-clamp recordings were performed on enzymatically isolated rat superior cervical sympathetic neurons. Results Midazolam dose-dependently blocked the whole-cell sodium currents evoked by a voltage step to 0 mV from a holding potential of -80 mV with a mean drug concentration required to produce 50% current inhibition (IC50) values of 18.35 ?mol?L-1; Clinically relevant concentration of midazolam(0.3 ?mol?L-1) reduced sodium peak currents by 19.98%(P
