Effect of total paeony glycoside(TPG) and its mechanism on calcium overloading injury in cultured PC12 cells
- VernacularTitle:赤芍总甙对氯化钾及N-甲基-D-门冬氨酸诱导的PC12细胞钙超载损伤的保护作用
- Author:
Li-Na HE
;
Jun YANG
;
Su-Bing HE
;
- Publication Type:Journal Article
- Keywords:
total paenony glycoside(TPG);
PC12 cell;
calcium overload;
KCl;
NMDA
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim The protective effect and mechanism of TPG on PC12 cells in calcium overloading injury models were studied. Methods Two injury models induced by KCl and NMDA were used to assay the action of TPG in cultured PC12 cells. Results The morphological examination revealed that TPG possessed obvious protective effects on PC12 cells in injury models. MTT and LDH measurement indicated that TPG increased the number of live cells and reduced the extent of cell injury significantly. TPG also lessened the concentration of calcium ion in cytoplasm. Conclusion TPG protected rat PC12 cells against two calcium overloading injuries effectively in vitro. Its actions may deal with anti oxidation, inhibition of NO production and blocking of both types of calcium channel.
