Comparative cardiotoxicity of propofol and thiopental in neonatal primary myocardial cell cultures
- VernacularTitle:异丙酚和硫喷妥钠对心肌细胞毒性作用的对比研究
- Author:
Tie-Zheng ZHANG
;
Feng-Xue WANG
;
Xiao-Jiang XIU
;
Chao-Ren WANG
;
- Publication Type:Journal Article
- Keywords:
propofol;
thiopental;
myocardium;
cytotoxicity;
cell culture
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the cardiotoxicity of propofol and thiopental. Methods 4day-old contracting neonatal primary myocardial cells obtained from 2-to 3-day-oldWistar rats were divided into 5 groups, with normal contrast group, and the cellcultures in groups PL, PH, TL and TH, were treated with propofol(3 ? 10-5 and3 ? 10-4 mol? L) and thiopental (1 ? 10-5 and 1 ? 10-4 mol?L) for 8 h.The con-tractility and morphology of the cells were observed and the cytoplasmic enzyme(LDH, AST, CK and ALP) release content of myocardial cell and the concentrationof electrolytes (K +, Na +, Cl - and Ca2+ ) in the medium were measured 8 h afterintravenous anesthetics administration. Results In groupPH and TL decreasedsignificantly (P
