Study on the mRNA expression of potassium channel gene Kv1-5 in the developing rat brain
- VernacularTitle:钾通道基因Kv 1.5在大鼠脑不同发育阶段的mRNA表达研究
- Author:
Yalan ZHANG
;
Xiaoliang WANG
;
- Publication Type:Journal Article
- Keywords:
potassium channel gene Kv 1 5;
mRNA expression;
rat;
brain
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the patterns of potassium channel gene Kv 1 5 mRNA expression in the developing rat brain by using RNase protection assay. METHODS (1) RNA was extracted from brain tissues by one step method, (2) The preparation of DNA template, (3) Synthesis of RNA probe in vitro transcription, (4) RNA:RNA. RESULTS Kv 1 5 was absent in embryo tissue, but significantly increased at postnatal day 25 with adult rat, the mRNA expression did not show any change. CONCLUSION there is close relationship between Kv 1 5 mRNA expression is pertinent to the development and function differentiation of rat brain.
