Detection of hepatitis C virus RNA in bone marrow mononuclear cells by digoxin-labeled in situ hybridization
- VernacularTitle:地高辛标记原位杂交技术检测骨髓单个核细胞中HCV RNA
- Author:
Qin XIA
;
Shuxian SHI
;
Deying TIAN
;
- Publication Type:Journal Article
- Keywords:
In situ hybridization;
Bone-marrow mononuclear cells;
HCV RNA
- From:
Chinese Journal of Infectious Diseases
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate HCV extrahepiatic infection and replication site as well as its relation with liver damage,bone-marrow mononuclear cells from 20 cases of nutreated chronic hepatitis C virus infect- ed patients were studied by digoxin-labeled in situ hybridization.Positive HCV RNA stained were de- tected in 18 of 20 cases (90%).Negative HCV RNA stained were not detected in any cases.The results suggested that there was HCV RNA in the bone-marrow of the patients with hepatitis C and bone-mar- row could be infected by HCV.But bone-marrow cell is not the HCV replication site.This study has some significance in HCV elucidating the pathogenicity of as well as the pathogenesis of chronic hepatitis C.
