STUDIES ON FACTORS INFLUENCING DOT-IGSS FOR ANTIBODIES IN SCHISTOSOMIASIS
- VernacularTitle:斑点免疫金银染色检测日本血吸虫抗体影响因素的探讨
- Author:
Wenping DU
;
Yisheng LIU
;
Yiwen RONG
;
Shuyun YUAN
;
- Publication Type:Journal Article
- Keywords:
dot-immunogold-silver staining (Dot-IGSS);
schistosoma japonicum;
antibody assay;
influential factor
- From:
Chinese Journal of Schistosomiasis Control
1989;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
By using different blocking and diluting fluids, different developing time and different gold-labeled anti-immunoglobulin (GLA-Ig) which had been preserved in different conditions, the influence on the dot-immunogold-siiver staining (Dot-IGSS) for diagnosis of schis-tosomiasis japonica was studied. The results suggested that using suitable amount of sheep/ bovine serum albumin (BSA) as blocking fluid and calf serum as diluting fluid was a good choice, and 20℃ 20 min was the appropriate time for development. The GLA-Ig without Na3N3 could be preserved in - 20℃ for 2 years if it was not frozen and melted repeatedly.
