STUDY ON ANTIBODY DEPENDENT CELI-MEDIATED CYTOTOXICITY OFBRUCIA MALAY I IN VITRO
- VernacularTitle:抗体依赖细胞介导对马来丝虫L_3细胞毒作用的体外研究
- Author:
Zeng FANG
;
Yaxiong XU
;
- Publication Type:Journal Article
- From:
Chinese Journal of Schistosomiasis Control
1989;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In vitro cellular adherence of murine neutrophils (Neu), macrophages (Mo) and eosinophils (Eos) to infective larvae (L3) of periodic Brugia malayi was promoted by immune serum (AIMS) or fresh normal mice serun (fNMS). Mo and Neu were more potent than Eos . in killing the L3. Eos could adhere to the L3 in the presence of AIMS or fNMS but a weak cy-totoxic effect was observed only when AIMS was used. The activity in AIMS could be removed with Staphylococcus aureus containing Protein-A (SPA) or anti-mice IgG antiserum suggesting that IgG might be in the observed cytotoxicity. Immunofluorescent staining revealed IgG binding to the surface of L3 after incubation with AIMS. Heat treatment of AIMS at 56℃ 30 min resulted in a reduced cytotoxic effect suggesting the role of complement in the reaction. EDTA but not EGTA abolished this activity indicating the operation of complement by alternate pathway. Scanning electron microscopic (SEM) studies comfirmed that the celluar adherence resulted in gross surface damage to the infective larvae.
