PREPARATION AND IMMUNOLOGICAL CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO B BLOOD GROUP SUBSTANCES
- VernacularTitle:抗B血型物质单克隆抗体的研制及其免疫学特性的研究
- Author:
Huatang CHEN
;
Xiaoqin WANG
;
Lingyun YUAN
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
B blood group substances;
Hybridoma technology;
Monoclonal antibody;
Enzyme-linked immunosorbent assay
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Using hybridoma technic, we prepared a group of hybridomas by different immunization procedures with(?)B erythrocytes, B erythrocytes plus B blood group substances or B blood group substances alone as antigens ,respectively. Three of them: 3-3-D9(IgG1) , 3-5-D1 2 (IgG1) and 6-1-G11 ( IgA ) which secreted high level and high specific monoclonal anti-B blood group substances were established. The agglutination titers of their tissue culture supernatant were 4096, 4096 and 16 384 by Takatsy microtitration assays. It was demonstrated that they were only specific to B blood group by hemagglutination specificity tests, hemagglutination inhibition assays and antibody dilution tests. They will be very useful in blood grouping to replace the human poly-clonal anti-B sera. Studies to determinate their combining site structures will be possible by these monoclonal antibodes.
