EFFECTS OF PODOPHYLLIC ACID PIPERIDYL HYDR-AZONE NITROXIDE RADICAL ON THE PROLIFERATION, MITOTIC INDEX AND DNA SYNTHESIS OF SOME CANCER CELLS IN VITRO
- VernacularTitle:鬼臼酰肼哌啶腙氮氧自由基和鬼臼乙叉甙对体外培养癌细胞增殖、有丝分裂指数和DNA合成的影响
- Author:
Junzhi WANG
;
Peiyan ZHANG
;
Zhongdong LIANG
;
- Publication Type:Journal Article
- Keywords:
Podophyllic acid piperidyl hydrazone nitroxide radical (GP_1);
VP_(l6);
Leukemia_(7712);
Leukemia_(1210);
sarcoma_(130);
Ascitic hepatoma;
(~3H) Thymidine;
Mitotic index
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
It was found that the proliferation of mice L7712 leukemic cells in vitro was markedly inhibited by 5 mg/L GP1 and VP10 The inhib itory rate increased with the incubation time. At a concentration of 0 .04-20mg/L, the mitotic index ( MI ) of GP1 group increased, but the MI of VP16 group decreased. After L7712 cells were treated with GP, 5 mg/L for 12 h the MI reached the highest point which was 8 times as high as that of the control, at the same time, the MI of VP16 ( 5 mg/L) group was about one-third of that of the control. The result of the combination of GP1 with VP16 showed that VP16 could antagonize the effect of GP on MI of L7712 cells. After being treated by GP1 and VP18 for 24 h, serious damage of L7712 cells could be observed. Both drugs inhibited the incorporation of (3H) TdR into DNA of S180, ascitic hepatoma (AH), L1210 and L7712 cells incubated for 24 h. It was further observed that S180 and L7712 cells were more sensitive than other cells to both drugs.
