DETERMINATION OF FOLIC ACID IN FORTIFIED INFANT MILK POWDER BY HPLC
- VernacularTitle:强化婴幼儿奶粉中叶酸的测定
- Author:
Xiaoning LI
;
Xiaoling LIU
;
- Publication Type:Journal Article
- Keywords:
folic acid fortified infant milk powder HPLC
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The fortified milk powder is dissolved in 0.05M K2HPO4 buffer solution, adjust pH to 4.5, and centrifuge for 20 minutes. The supernatant is then adjusted to pH 4.0, go through CGS resin column using 10ml KC1-K2HPO4 as eluant. The eluate is collected as testing solution and injected to HPLC. Column: ODS; mobile phase; 0.05M K2HPO4 : CH3OH (91:9); ultraviolet detector: 285nm. CV% is 3.3% and recovery 87%.
