Comet Assay with Vicia faba Root Cells
- VernacularTitle:蚕豆根尖细胞彗星实验研究
- Author:
Qing-Bi ZHANG
;
Zhong-Lin GAN
;
- Publication Type:Journal Article
- Keywords:
DNA damage;
Viciafaba root cells;
Comet assay
- From:
Journal of Environment and Health
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a sensitive,quick and convenient comet assay with Viciafaba root cells,so as to provide the reference for biomonitoring in situ.Methods The K_2CrO_7 and H_2O_2 were used as the positive control,the Viciafaba root cells isolated with mechanical and enzyme treatment respectively were exposed to the different concentrations of K_2CrO_7(1 nmol/L,10 nmol/L,100 nmol/L,1000 nmol/L)and H202(25 ? mol/L,50 ? mol/L,100 ?mol/L,200 ?mol/L).The effect of DNA damage was detected by using comet assay.Results The yield of Viciafaba root cells isolated from enzyme treatment were much higher than that from mechanical treatment.K_2CrO_7and H_2O_2 could cause DNA damage in Viciafaba root cells,there was significant difference between exposure group and control group(P
