Rapid Screening Method for Ten Sedative-hypnotica Drug in Functional Food by Liquid Chromatography with Mass Spectrometry
- VernacularTitle:保健品中安眠镇定类药物的高效液相色谱-质谱筛选分析
- Author:
Xi-Mei WU
;
Bing-Hui ZHU
;
Ming CHEN
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Chromatography,liquid;
Mass spectroscopy;
Sedative hypnotica drug;
Functional food
- From:
Journal of Environment and Health
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for the simultaneous separation and determination of 10 kinds of sedative hypnotica drugs in the functional food with high performance liquid chromatography mass spectrometry system.Methods The mobile phase consisted of acetonitrile and 15 mmol/L ammonium acetate solution(0.1% formic acid ),chromatographic column was Zobax SB C 18.Identification was based on the compound's absolute retention time,protonated molecular ion,and representative fragment ion by multiple reaction monitoring.The condition of determination was investigated and optimized.Results With this method,the linear range for the 10 drugs was 10-1 080 ?g/kg,the average recoveries ranged from 80.5%-97.1% and the detection limits were from 0.35-12.0?g/kg respectively.Conclusion The method established in the present paper is applicable to monitoring sedative hypnotica drug in the functional food.
